Based on constants in the table, provide the order that a mixture of proteins would elute on an anion exchange column with a pH of 7 elution buffer? Can you please explain the answer thank you!

A mixture of four proteins with their charges in a phosphate buffer of pH 7.4 are as follows: A, B+1, C+2, D-1.The protein ____ will bind strongly to the matrix of an anion exchange column

The pI of a protein is 6.5. Which of the following buffers should be used so that the protein could bind to a cation exchange column for purification

Which change in solution composition would cause a protein to elute from a hydrophobic interaction column?A.Decreasing pHB.Increasing pHC.Decreasing salt concentrationD.Increasing salt concentration

A buffer is a solution that ______.Multiple choice question.resists changes in pH when limited amounts of acid or base are addedchanges color when limited amounts of acid or base are added.always has a pH of 7, even when limited amounts of acid or base are addedquickly changes pH when limited amounts of acid or base are added

Arrange the following in the increasing order of pH values according to givenpH scale.A. NaOH solution B. BloodC. Lemon juice D. Milk of magnesia(a) C < B < D < A (b) A < B < C < D(c) D < C < B < A (d) A < B < D < C