A mixture of four proteins with their charges in a phosphate buffer of pH 7.4 are as follows: A, B+1, C+2, D-1.The protein ____ will bind strongly to the matrix of an anion exchange column
Question
A mixture of four proteins with their charges in a phosphate buffer of pH 7.4 are as follows: A, B+1, C+2, D-1.The protein ____ will bind strongly to the matrix of an anion exchange column
Solution
The protein that will bind strongly to the matrix of an anion exchange column is protein C. This is because anion exchange columns attract and bind molecules that are negatively charged. However, in this case, all proteins are either neutral or positively charged. Among them, protein C has the highest positive charge (+2), and it will be the last one to be eluted from the column. Therefore, it can be said that protein C will bind most strongly to the anion exchange column.
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