select one or more statement correctly describe how inner cells can be distinguished from outer cells in the pre-implantation mouse embryo Outer cells are sealed via zippering of actin rings, inner cells are not sealed. Hippo signalling is off in the outer cells, but on in the inner cells.Outer cells are pluripotent (= giving rise to any organ of the adult body), inner cells are determined to give rise to extraembryonic tissue only Inner cells have a “contact-free” surface, outside cells are entirely surrounded by neighbour cells.

"Outer cells are sealed via zippering of actin rings, inner cells are not sealed." - This statement is TRUE. The outer cells of the pre-implantation mouse embryo, also known as the trophectoderm, are sealed by tight junctions, which are formed by the zippering of actin rings. The inner cells, or the inner cell mass, are not sealed in this way.

"Hippo signalling is off in the outer cells, but on in the inner cells." - This statement is TRUE. The Hippo signaling pathway is known to be active in the inner cells of the pre-implantation mouse embryo, where it helps to regulate cell proliferation and differentiation. In the outer cells, Hippo signaling is typically inactive.

"Outer cells are pluripotent (= giving rise to any organ of the adult body), inner cells are determined to give rise to extraembryonic tissue only" - This statement is FALSE. It's actually the opposite. The inner cells, or the inner cell mass, are pluripotent and can give rise to all cell types in the adult body. The outer cells, or the trophectoderm, are determined to give rise to the placenta and other extraembryonic tissues.

"Inner cells have a “contact-free” surface, outside cells are entirely surrounded by neighbour cells." - This statement is TRUE. The inner cells of the pre-implantation mouse embryo have at least one surface that is not in contact with other cells, while the outer cells are completely surrounded by neighboring cells.