What is the net charge of the peptide KENLVMNDT at pH7?B) What kind of ion exchange resin would you use to bind the peptide at pH7?C) What is the net charge of the peptide at pH12?

At pH 7, approximately what charge would be on your peptide? Explain your answer. (2 marks)

A synthetic peptide contains 4 negatively charged and 8 positively charged amino acids at pH 6.1. When the peptides are treated with an alkaline solution having a pH of 8.9, 4 positively charged groups become deprotonated. At pH 8.9, what is the net charge on that peptide? What will be the direction of movement (towards cathode or anode) if the alkali treated peptides are now placed in an electric filed? 0; Movement of the peptide will not happen -8; Movement of the peptide will be towards anode +12; Movement of the peptide will be towards anode -4; Movement of the peptide will be towards cathode

What is the net charge on the amino acid lysine at pH = 7.4 if pKa1 = 2.16, pKa2 = 9.06, and the pKa of the side chain = 10.54?A.+1B.+2C.0D.-1

A mixture of four proteins with their charges in a phosphate buffer of pH 7.4 are as follows: A, B+1, C+2, D-1.The protein ____ will bind strongly to the matrix of an anion exchange column

Which amino acid substitution would result in the greatest change in the net charge of a polypeptide at pH 7?A.Glu to LysB.Asp to AlaC.Lys to ArgD.Arg to Ser