An investigator keeps her mice in a room with LD12:12 with lights on at 6 am. She wants to change the lights to a skeleton photoperiod (1 h pulses) without changing the animals' entrainment. What schedule would accomplish her goal?
Question
An investigator keeps her mice in a room with LD12:12 with lights on at 6 am. She wants to change the lights to a skeleton photoperiod (1 h pulses) without changing the animals' entrainment. What schedule would accomplish her goal?
Solution
The investigator can accomplish her goal by setting the lights to turn on for one hour at the time the mice are used to waking up, and then again for one hour when they are used to going to sleep.
In this case, since the mice are used to a 12:12 light-dark cycle with lights on at 6 am, the investigator should set the lights to turn on from 6 am to 7 am, and then again 12 hours later, from 6 pm to 7 pm. This would maintain the animals' entrainment while changing to a skeleton photoperiod.
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