You would like to sterilize bacterial growth media in a flask. You have about 1.5 hours before your patient’s sample will be ready for inoculation. Which method would be best to sterilize the media within the timeframe?ANSWERPasteurizationDry heatAutoclavingBoiling for 15 minutes

Which of the following methods would be best for sterilizing a sample of a heat-sensitive solution?ANSWERUV radiationAutoclavingFiltrationFreezing

Dry Heat Sterilization

How should the inoculating loop be sterilised before using it to transfer bacteria to the Petri dish?Place it in the autoclaveDip it in the agar jellyHold it in the Bunsen flamePlace it in the nutrient broth

When growing bacteria in the laboratory, why is it a good idea to work next to a Bunsen flame?To sterilise the Petri dishTo melt the agarTo encourage bacteria to grow in the agar dishTo prevent airborne bacteria contaminating the agar dish2How should the inoculating loop be sterilised before using it to transfer bacteria to the Petri dish?Place it in the autoclaveDip it in the agar jellyHold it in the Bunsen flamePlace it in the nutrient broth3In the school laboratory, at what temperature should the bacteria be incubated?37°C10°C25°C50°C4Paper discs containing different antibiotic solutions are placed on an agar plate covered in bacteria. How could you compare the effectiveness of these antibiotics on bacterial growth after several days?Measure the size of each discObserve the colour change around each discMeasure the area of the inhibition zone around each discObserve the temperature change around each disc

Match each sterilization method with its definition or description.-Steam-Dry Heat-Filtration-Gas-Ionizing RadiationA. the best terminal sterilization method, all other methods are compared to this methodB. containers, products in containers are sterilized by exposure to various raysC. oxidizing agents used to kill microbes in packaged supplies, on tools & equipment, and in products, some drugs are sterilized this wayD. for products that cannot withstand heat, removes living and dead microbes and particulates larger than a nominal pore sizeE. high heat, not for aqueous liquids, destroys pyrogens, penetrates petroleum jelly ointments