How should the inoculating loop be sterilised before using it to transfer bacteria to the Petri dish?Place it in the autoclaveDip it in the agar jellyHold it in the Bunsen flamePlace it in the nutrient broth
Question
How should the inoculating loop be sterilised before using it to transfer bacteria to the Petri dish?Place it in the autoclaveDip it in the agar jellyHold it in the Bunsen flamePlace it in the nutrient broth
Solution
The correct method to sterilise the inoculating loop before using it to transfer bacteria to the Petri dish is to hold it in the Bunsen flame. Here are the steps:
- Turn on the Bunsen burner.
- Hold the handle of the inoculating loop.
- Place the loop part of the inoculating loop in the flame of the Bunsen burner.
- Keep the loop in the flame until it glows red hot. This ensures that any bacteria or other microorganisms on the loop are killed.
- Allow the loop to cool before using it to transfer bacteria. This is to prevent the heat from the loop killing the bacteria that you are about to transfer.
- Once cooled, the loop is now sterilised and ready to use.
Similar Questions
When growing bacteria in the laboratory, why is it a good idea to work next to a Bunsen flame?To sterilise the Petri dishTo melt the agarTo encourage bacteria to grow in the agar dishTo prevent airborne bacteria contaminating the agar dish2How should the inoculating loop be sterilised before using it to transfer bacteria to the Petri dish?Place it in the autoclaveDip it in the agar jellyHold it in the Bunsen flamePlace it in the nutrient broth3In the school laboratory, at what temperature should the bacteria be incubated?37°C10°C25°C50°C4Paper discs containing different antibiotic solutions are placed on an agar plate covered in bacteria. How could you compare the effectiveness of these antibiotics on bacterial growth after several days?Measure the size of each discObserve the colour change around each discMeasure the area of the inhibition zone around each discObserve the temperature change around each disc
Select ALL of the following statements that are INCORRECT regarding microbiology lab procedures Group of answer choices For safety’s sake the Bunsen burner should be left sitting underneath the gas taps or gas tubing. To sterilise a loop you need to hold it in the bunsen burner until it glows red hot. Always drain the ethanol off the spreader or forcepts before flaming to avoid dripping burning ethanol on anything. Set up your work area in a manner that enables you to safely use the space without reaching across the Bunsen burner. When flaming the spreader or the forceps, hold the spreader tilted down so that any flaming ethanol runs away from your hands. It is safe to hold any flaming instrument over the ethanol beaker.
You would like to sterilize bacterial growth media in a flask. You have about 1.5 hours before your patient’s sample will be ready for inoculation. Which method would be best to sterilize the media within the timeframe?ANSWERPasteurizationDry heatAutoclavingBoiling for 15 minutes
Imagine that you forgot to flame the loop before streaking the inoculum from the first quadrant into the second quadrant. What is the most likely consequence of this error?Too much bacterial growth outside the first quadrant.Contamination of the broth culture.Too little bacterial growth outside the first quadrant.Contamination of the Petri plate culture.
Dry Heat Sterilization
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