When growing bacteria in the laboratory, why is it a good idea to work next to a Bunsen flame?To sterilise the Petri dishTo melt the agarTo encourage bacteria to grow in the agar dishTo prevent airborne bacteria contaminating the agar dish2How should the inoculating loop be sterilised before using it to transfer bacteria to the Petri dish?Place it in the autoclaveDip it in the agar jellyHold it in the Bunsen flamePlace it in the nutrient broth3In the school laboratory, at what temperature should the bacteria be incubated?37°C10°C25°C50°C4Paper discs containing different antibiotic solutions are placed on an agar plate covered in bacteria. How could you compare the effectiveness of these antibiotics on bacterial growth after several days?Measure the size of each discObserve the colour change around each discMeasure the area of the inhibition zone around each discObserve the temperature change around each disc
Question
When growing bacteria in the laboratory, why is it a good idea to work next to a Bunsen flame?To sterilise the Petri dishTo melt the agarTo encourage bacteria to grow in the agar dishTo prevent airborne bacteria contaminating the agar dish2How should the inoculating loop be sterilised before using it to transfer bacteria to the Petri dish?Place it in the autoclaveDip it in the agar jellyHold it in the Bunsen flamePlace it in the nutrient broth3In the school laboratory, at what temperature should the bacteria be incubated?37°C10°C25°C50°C4Paper discs containing different antibiotic solutions are placed on an agar plate covered in bacteria. How could you compare the effectiveness of these antibiotics on bacterial growth after several days?Measure the size of each discObserve the colour change around each discMeasure the area of the inhibition zone around each discObserve the temperature change around each disc
Solution
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When growing bacteria in the laboratory, it is a good idea to work next to a Bunsen flame to prevent airborne bacteria contaminating the agar dish. The heat from the flame creates an updraft that pushes airborne particles away from your workspace.
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The inoculating loop should be sterilised before using it to transfer bacteria to the Petri dish by holding it in the Bunsen flame. This ensures that no unwanted bacteria are transferred to the Petri dish.
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In the school laboratory, the bacteria should be incubated at 37°C. This is the optimal temperature for bacterial growth as it is close to the human body temperature, which is where many bacteria naturally thrive.
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To compare the effectiveness of different antibiotic solutions on bacterial growth after several days, you should measure the area of the inhibition zone around each disc. The larger the inhibition zone, the more effective the antibiotic is at preventing bacterial growth.
Similar Questions
How should the inoculating loop be sterilised before using it to transfer bacteria to the Petri dish?Place it in the autoclaveDip it in the agar jellyHold it in the Bunsen flamePlace it in the nutrient broth
Select ALL of the following statements that are INCORRECT regarding microbiology lab procedures Group of answer choices For safety’s sake the Bunsen burner should be left sitting underneath the gas taps or gas tubing. To sterilise a loop you need to hold it in the bunsen burner until it glows red hot. Always drain the ethanol off the spreader or forcepts before flaming to avoid dripping burning ethanol on anything. Set up your work area in a manner that enables you to safely use the space without reaching across the Bunsen burner. When flaming the spreader or the forceps, hold the spreader tilted down so that any flaming ethanol runs away from your hands. It is safe to hold any flaming instrument over the ethanol beaker.
You would like to sterilize bacterial growth media in a flask. You have about 1.5 hours before your patient’s sample will be ready for inoculation. Which method would be best to sterilize the media within the timeframe?ANSWERPasteurizationDry heatAutoclavingBoiling for 15 minutes
Which of the following is not a method of dry heat sterilization FlamingIncinerationPasteurizationHot air oven
The images below show the main steps involved when investigating the effect of antiseptics on the growth of bacteria.Place the images in the correct order.Step 1Step 2Step 3Step 4Step 5Step 6Possible AnswersI DON'T KNOWRESETSUBMIT ANSWERLearning MaterialChoose your materialCENTURY ExplainsSlideshowAim & BackgroundVariablesEquipment IEquipment IIAutoclave & IncubatorSafetyResults TableCalculator FunctionMethod: Clean the Work SurfaceMethod: Bunsen BurnerMethod: Agar Plate LabelsMethod: ControlMethod: Disc 1Method: Disc LocationMethod: Control DiscMethod: Seal with TapeAnalysis: Zone of InhibitionAnalysis: Mean Zone MeasurementsAnalysis: Calculate Mean Cross-sectional AreaConclusionAlternative Method IAlternative Method IIQuestionQuestionQuestionQuestionQuestionQuestionQuestionQuestionQuestionQuestionQuestionQuestionSummary
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