Select ALL of the following statements that are INCORRECT regarding microbiology lab procedures Group of answer choices For safety’s sake the Bunsen burner should be left sitting underneath the gas taps or gas tubing. To sterilise a loop you need to hold it in the bunsen burner until it glows red hot. Always drain the ethanol off the spreader or forcepts before flaming to avoid dripping burning ethanol on anything. Set up your work area in a manner that enables you to safely use the space without reaching across the Bunsen burner. When flaming the spreader or the forceps, hold the spreader tilted down so that any flaming ethanol runs away from your hands. It is safe to hold any flaming instrument over the ethanol beaker.

When growing bacteria in the laboratory, why is it a good idea to work next to a Bunsen flame?To sterilise the Petri dishTo melt the agarTo encourage bacteria to grow in the agar dishTo prevent airborne bacteria contaminating the agar dish2How should the inoculating loop be sterilised before using it to transfer bacteria to the Petri dish?Place it in the autoclaveDip it in the agar jellyHold it in the Bunsen flamePlace it in the nutrient broth3In the school laboratory, at what temperature should the bacteria be incubated?37°C10°C25°C50°C4Paper discs containing different antibiotic solutions are placed on an agar plate covered in bacteria. How could you compare the effectiveness of these antibiotics on bacterial growth after several days?Measure the size of each discObserve the colour change around each discMeasure the area of the inhibition zone around each discObserve the temperature change around each disc

What action is NOT necessary before lighting a Bunsen burner?Select one:Clear the burner area of flammable materials such as solvents and papers.Check that the gas regulator and air inlet on the burner can be adjusted.Make sure there are no holes in the hose connected to the burner.Leave the gas cock open for a few minutes before lighting to clear the line.

What precautions should be taken whilst using a bunsen burner?Group of answer choicesEnsure loose clothing (eg long sleeves) are tied back (or not worn).The bunsen burner is only used for it's intended purpose in the experiment.Ensure loose paper sheets (which could be easily blown with a breeze) are not nearby the bunser burnerLong hair should be tied back to avoid falling into the flame.Ensure there are no flammable chemicals nearby (eg organic solvents)

How should the inoculating loop be sterilised before using it to transfer bacteria to the Petri dish?Place it in the autoclaveDip it in the agar jellyHold it in the Bunsen flamePlace it in the nutrient broth

Which of the following is a proper lab procedure? asetting your lab equipment as close to the edge of the table as possible to minimize reaching bleaving your sleeves around your wrists cleaving your Bunsen burner lit while you leave your table dusing beaker tongs to pick up a hot beaker