Which of the following would be most effective for sequencing the exact 5’- end of an mRNAtranscript?A. PCRB. Primer extensionC. CloningD. Southern blotE. Linkage mapping

Which important nucleotide sequence is found upstream (5’) of a gene’s transcription start site?Question 4Answera.The -10 and -35 regionsb.The terminatorc.The coding sequenced.The ATG start codon

Below is a sequence of DNA that is going to be replicated. This is the sequence of the leading strand and only shows the position at which primase binds to synthesise RNA primers. Based on this sequence, which RNA primers would allow replication from both the leading and lagging strands?5’ ….TTATCGCTGA….AGAGGACGTA….CATTGACAGG 3’Group of answer choicesFrom the leading strand: 5’ UUAUCGCUGA 3’ and 5’ AGAGGACGUA 3’From the lagging strand: 5’ CCUGUCAAUG 3’From the leading strand: 5’ CAUUGACAGG 3’From the lagging strand: 5’ UCAGCGAUAA 3’ and 5’ UACGUCCUCU 3’From the leading strand: 5’ UCAGCGAUAA 3’ and 5’ UACGUCCUCU 3’From the lagging strand: 5’ CAUUGACAGG 3’From the leading strand: 5’ CCUGUCAAUG 3’From the lagging strand: 5’ UUAUCGCUGA 3’ and 5’ AGAGGACGUA 3’

Which of the following mRNA strands contains the longest open reading frame?A.5′-CCACGAUGCGUUGAGCGC-3′B.5′-CAAUGGUAAAGUCUUAGU-3′C.5′-GACGAUGUAACUUGCACU-3′D.5′-AGAAUGGUAUCCUGAGCC-3′

Using the coding strand below, make a template strand and transcribe to mRNA.Then, translate the mRNA into a protein (note: Indicate the 5 prime ends and 3 primeends in your template and mRNA strand. In your protein, indicate the amino terminaland carboxyl terminal)5’-----ATGGGGCTCAGCGAC----- 3’Template:mRNA:Protein:2. Encircle the correct lettered answer: Transcription of the following sequence of atryptophan operon occurs in the direction indicated by the arrow. What would be thebase sequence of the mRNA produced?3’-----CGCCGCTGCGCG ---- 5’Transcription  Which product?5’-----GCGGCGACGCGC----- 5’19A. 5’-----GCGGCGACGCGC ---- 3’B. 5’-----GCGCGUCGCCGC ---- 3’C. 5’-----GCGCGTGCGGCG ---- 3’D. 5’-----GCGGCGUCGCGC ---- 3’E. 5’-----CGCGCTCGCCGC ----- 3’3. Encircle the correct letter answer: The base sequence of codons 57-58 in thecytochrome Beta-5 reductase gene is CAGCGC. The mRNA produced upontranscription of this gene will contain the sequence:A. GCGCTGB. CUGCGCC. GCGCUGD. CAGCGCE. GUCGCG4. Refer the normal coding strand below:ATG GCA AAT CGT TTT TTA CCT ATA GGGUsing the genetic code as your guide, illustrate coding strands that show a silentmutation, a missense mutation, a nonsense mutation, and a frameshift mutation.Silent mutation:Missense mutation:Nonsense mutation:Frameshift mutation:20First position (5’ End) Second position Third position (3’ End)U C A GUPhe Ser Tyr Cys UPhe Ser Tyr Cys CLeu Ser STOP STOP ALeu Ser STOP Trp GCLeu Pro His Arg ULeu Pro His Arg CLeu Pro Gln Arg ALeu Pro Gln Arg GAIle Thr Asn Ser UIle Thr Asn Ser CIle Thr Lys Arg AMet Thr Lys Arg GGVal Ala Asp Gly UVal Ala Asp Gly CVal Ala Glu Gly AVal Ala Glu Gly G5. Refer to the pedigree below:21Is this trait dominant or recessive? Explain your answer.Is this trait autosomal or X-linked? Explain your answer.Give examples of diseases that exhibit this mode of inheritance.6. Illustrate a three-generation pedigree showing mitochondrial inheritance. Give examples

Consider a given stretch of mRNA sequence. Which of the following shall you consider singe stranded DNA probe for the given mRNA sequence? 3' TGAACCTGAGTA 5' 5' TGAACCTGAGTA 3' 3' UGAACCUGAGUA 5' 5' UGAACCUGAGUA 3'